simpleprobe ® probes  (TIB MOLBIOL)

Journal: Journal of Clinical Microbiology

Article Title: Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR

doi: 10.1128/JCM.02217-20

Figure Lengend Snippet: Sequences of oligonucleotides relevant to this study

Article Snippet: The resulting PCR product was detected postamplification using 3 SimpleProbe probes for the identification of Bartonella genus, B. henselae , and B. quintana DNA (TIB MOLBIOL, Berlin, Germany, and Dyn Diagnostics, Migdal HaEmeq, Israel) ( ).

Techniques: Sequencing, Real-time Polymerase Chain Reaction

Journal: Journal of Clinical Microbiology

Article Title: Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR

doi: 10.1128/JCM.02217-20

Figure Lengend Snippet: Multiplex real-time PCR using 3 SimpleProbe probes: Bartonella genus-specific, B. henselae-specific, and B. quintana-specific probes. PCR was performed on valvular tissue of a 65-year-old patient with native aortic valve Bartonella quintana endocarditis (patient number 34 [Table 2]). Two fluorescent peaks corresponding to melting temperatures (Tms) of 57.2°C and 67.6°C are the result of detachment of the genus-specific and B. quintana-specific probes from the PCR product, respectively. Plasmids pMR-ribC185-Bq and pMR-ribC185-Bh were used as positive controls for B. quintana and B. henselae, respectively.

Article Snippet: The resulting PCR product was detected postamplification using 3 SimpleProbe probes for the identification of Bartonella genus, B. henselae , and B. quintana DNA (TIB MOLBIOL, Berlin, Germany, and Dyn Diagnostics, Migdal HaEmeq, Israel) ( ).

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction

Journal: Journal of Clinical Microbiology

Article Title: Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR

doi: 10.1128/JCM.02217-20

Figure Lengend Snippet: Multiplex real-time PCR using 2 SimpleProbe probes, B. henselae-specific and genus-specific probes. PCR was performed on valvular tissue of a 56-year-old patient with native mitral valve Bartonella henselae endocarditis (patient number 13 [Table 2]). Two fluorescent peaks corresponding to Tms of 49.3°C and 59.8°C are the result of detachment of the B. henselae-specific and genus-specific probes from the PCR product, respectively. Plasmids pMR-ribC185-Bq and pMR-ribC185-Bh were used as positive controls for B. quintana and B. henselae, respectively. The peak corresponding to the B. quintana-specific probe is not seen since the B. quintana-specific probe was not used in this specific assay.

Article Snippet: The resulting PCR product was detected postamplification using 3 SimpleProbe probes for the identification of Bartonella genus, B. henselae , and B. quintana DNA (TIB MOLBIOL, Berlin, Germany, and Dyn Diagnostics, Migdal HaEmeq, Israel) ( ).

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Development of a SimpleProbe real-Time PCR Assay for rapid detection and identification of the US novel urethrotropic clade of Neisseria meningitidis ST-11 (US_NmUC)

doi: 10.1371/journal.pone.0228467

Figure Lengend Snippet: Amplification curves generated when the SimpleProbe® US_NmUC assay was tested against various microorganisms that can colonize the urogenital tract.

Article Snippet: A SimpleProbe® probe PNM1 ( Fluorescein-SPC-CGTCATCAGCGATACGCG-Phosphate ) was synthesized by FLUORESENTRIC (Park City, UT).

Techniques: Amplification, Generated

Journal: PLoS ONE

Article Title: Development of a SimpleProbe real-Time PCR Assay for rapid detection and identification of the US novel urethrotropic clade of Neisseria meningitidis ST-11 (US_NmUC)

doi: 10.1371/journal.pone.0228467

Figure Lengend Snippet: Amplification curves generated when the SimpleProbe® US_NmUC assay was tested against various dilutions of NG and NM.

Article Snippet: A SimpleProbe® probe PNM1 ( Fluorescein-SPC-CGTCATCAGCGATACGCG-Phosphate ) was synthesized by FLUORESENTRIC (Park City, UT).

Techniques: Amplification, Generated

Journal: PLoS ONE

Article Title: Development of a SimpleProbe real-Time PCR Assay for rapid detection and identification of the US novel urethrotropic clade of Neisseria meningitidis ST-11 (US_NmUC)

doi: 10.1371/journal.pone.0228467

Figure Lengend Snippet: Amplification curves generated from specimens of the first 20 men in the IUMP cohort using the SimpleProbe® US_NmUC assay.

Article Snippet: A SimpleProbe® probe PNM1 ( Fluorescein-SPC-CGTCATCAGCGATACGCG-Phosphate ) was synthesized by FLUORESENTRIC (Park City, UT).

Techniques: Amplification, Generated